Interactions between *Mycoplasma mycoides* subsp. *mycoides* and bovine macrophages under physiological conditionsuse asterix (*) to get italics

Philippe Totté, Tiffany Bonnefois, Lucia Manso-SilvanPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2023

<p style="text-align: justify;">Abstract</p> <p>We investigated the interactions of unopsonized and opsonized *Mycoplasma mycoides* subsp. *mycoides* (Mmm) with bovine macrophages *in vitro*. Mmm survived and proliferated extracellularly on bovine macrophage cell layers in the absence of Mmm-specific antisera. Bovine complement used at non-bactericidal concentrations did neither have opsonizing effect nor promoted intracellular survival, whereas Mmm-specific antisera substantially increased phagocytosis and Mmm killing. A phagocytosis-independent uptake of Mmm by macrophages occurred at high MOIs, also found to induce the production of TNF, and both responses were unaffected by non-bactericidal doses of bovine complement. Bovine complement used at higher doses killed Mmm in cell-free cultures and completely abrogated TNF responses by macrophages. These results provide a framework to identify Mmm antigens involved in interactions with macrophages and targeted by potentially protective antibodies and point towards a pivotal role of complement in the control of inflammatory responses in contagious bovine pleuropneumonia.</p>

https://doi.org/10.5281/zenodo.7442581You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

Contagious bovine pleuropneumonia, Mycoplasma mycoides, macrophages, complement, TNF

Microbe-microbe and microbe-host interactions

Flavio Sacchini f.sacchini@izs.it, Joachim Frey <joachim.frey@vetsuisse.unibe.ch>, GAURIVAUD Patrice Patrice.GAURIVAUD@anses.fr, Paola Pilo <paola.pilovetsuisse.unibe.ch>, Jose Perez-Casal <jose.perez-casal@usask.ca>, Jochen Meens Jochen.Meens@tiho-hannover.de No need for them to be recommenders of PCIMicrobiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2022-12-09 15:12:53

Pablo Zunino

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