The bacterial microbiome of symbiotic and menthol-bleached polyps of long-term aquarium-reared *Galaxea fascicularis*use asterix (*) to get italics

Giulia Puntin, Jane C.Y. Wong, Till Roethig, David M. Baker, Michael Sweet, Maren ZieglerPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2024

<p>Coral reefs support the livelihood of half a billion people but are at high risk of collapse due to the vulnerability of corals to climate change and local anthropogenic stressors. While understanding coral functioning is essential to guide conservation efforts, research is challenged by the complex nature of corals. They exist as metaorganisms (holobionts), constituted by the association between the (coral) animal host, its obligate endosymbiotic algae (Symbiodiniaceae), and other microorganisms comprising bacteria, viruses, archaea, fungi and other protists. Researchers therefore increasingly turn to model organisms to unravel holobiont complexity, dynamics, and how these determine the health and fitness of corals. The coral <em>Galaxea fascicularis</em>&nbsp;is an emerging model organism for coral symbiosis research with demonstrated suitability to aquarium rearing and reproduction, and to manipulation of the host-Symbiodiniaceae symbiosis. However, little is known about the response of the <em>G. fascicularis</em> microbiome to menthol bleaching—the experimental removal of the Symbiodiniaceae which represents the first step in coral-algal symbiosis manipulation. For this, we characterized the bacterial microbiome of symbiotic and menthol-bleached <em>G. fascicularis</em> originating from the Red Sea and South China Sea (Hong Kong) that were long-term aquarium-reared in separate facilities. We found that the coral-associated microbiomes were composed of relatively few bacterial taxa (10-78 ASVs). Symbiotic polyps (clonal replicates) from the same colony had similar microbiomes, which were distinct from those of other colonies despite co-culturing in shared aquaria. A pattern of seemingly differential response of the bacterial microbiome to menthol bleaching between the two facilities emerged, warranting further investigation into the role of rearing conditions. Nevertheless, the changes in community composition overall appeared to be stochastic suggesting a dysbiotic state. Considering the importance of bleaching treatment of captive corals for symbiosis research, our results—although preliminary—contribute fundamental knowledge for the development of the Galaxea model for coral symbiosis research.</p>

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA947274/You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

https://doi.org/10.5281/zenodo.10551928You should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://

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coral, model organism, 16S, holobiont, symbiosis, aquarium, ex situ

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Microbial symbiosis, Microbiomes

Robert Quinn, quinnrob@msu.edu, Cédric Hubas, cedric.hubas@mnhn.fr , Debashish Bhattacharya, d.bhattacharya@rutgers, Phillip Cleves, cleves@carnegiescience.edu, Daniel Tamarit suggested: Lisa Klasson (Lisa.klasson@icm.uu se), Jennah Dharamshi (Jennah.dharamshi@su.se), Elizabeth Hambleton [elizabeth.hambleton@univie.ac.at] suggested: Bettina Glasl, bettina.glasl@univie.ac.at No need for them to be recommenders of PCIMicrobiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2023-08-26 04:50:01

Yui Sato