Diversity of bacterial symbionts associated with the tropical plant bug *Monalonion velezangeli* (Hemiptera: Miridae) revealed by high-throughput 16S-rRNA sequencinguse asterix (*) to get italics

Lucio Navarro-Escalante, Pablo Benavides, Flor Edith AcevedoPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

2023

<p>Insects and microbes have developed complex symbiotic relationships that evolutionarily and ecologically play beneficial roles for both, the symbiont and the host. In most Hemiptera insects, bacterial symbionts offer mainly nutritional, defensive, and reproductive roles in addition to promoting the adaptive radiation of several hemipteran phytophagous lineages. The tropical plant bug <em>Monalonion</em> <em>velezangeli</em> (Hemiptera: Miridae) is a polyphagous herbivore considered an important insect pest for several economically relevant tropical crops, but information about the composition of its bacterial microbiota was missing. In this study, we describe the diversity and structure of the bacterial microbiota in the nymph and adult life stages of <em>M. velezangeli </em>using Illumina high-throughput sequencing of 16S ribosomal RNA gene amplicons (meta-barcoding). We found that both insect life stages share a similar microbiota in terms of bacterial diversity and community structure. The intracellular symbiont <em>Wolbachia</em> dominated the overall microbiome composition (~92%) in these life stages. Members of the core microbiota include <em>Wolbachia</em>, <em>Romboutsia</em>, <em>Ignavibacterium</em>, <em>Clostridium</em>, <em>Allobaculum</em>, <em>Paracoccus</em>, <em>Methylobacterium</em>, <em>Faecalibacterium</em>, <em>Collinsella</em>, <em>Rothia</em>, <em>Sphingomonas</em> and 4 other undetermined bacterial genera. Based on PCR screening and DNA sequencing of the wspgene, <em>Wolbachia</em> infection was confirmed in almost 80% of samples, and represented by two different isolates or strains within the supergroup B. This data offer opportunities for studying the contribution of symbiotic bacteria in the biological performance of this insect pest, and provides a base to explore other insect control methods.</p>

http://www.ncbi.nlm.nih.gov/bioproject/875474You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

bacterial microbiota, endosymbiont, plant bug, Monalonion

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Microbial ecology and environmental microbiology, Microbial symbiosis

Dr. Aileen Berasategui-Lopez aileen.berasategui-lopez@uni-tuebingen.de, Dr. Simone de Souza Prado simone.prado@embrapa.br, Dr. Hongwei Shan shanhongwei@nbu.edu.cn No need for them to be recommenders of PCIMicrobiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

2022-10-31 20:31:54

Jean-Marie Volland