PCI Microbiol

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Title *

Interactions between *Mycoplasma mycoides* subsp. *mycoides* and bovine macrophages under physiological conditionsuse asterix (*) to get italics

Authors *

Philippe Totté, Tiffany Bonnefois, Lucia Manso-SilvanPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year *

2023

Picture *

Abstract *

<p style="text-align: justify;">We investigated the interactions of unopsonized and opsonized *Mycoplasma mycoides* subsp. *mycoides* (Mmm) with bovine macrophages *in vitro*. Mmm survived and proliferated extracellularly on bovine macrophage cell layers in the absence of Mmm-specific antisera. Bovine complement used at non-bactericidal concentrations did neither have opsonizing effect nor promoted intracellular survival, whereas Mmm-specific antisera substantially increased phagocytosis and Mmm killing. A phagocytosis-independent uptake of Mmm by macrophages occurred at high MOIs, also found to induce the production of TNF, and both responses were unaffected by non-bactericidal doses of bovine complement. Bovine complement used at higher doses killed Mmm in cell-free cultures and completely abrogated TNF responses by macrophages. These results provide a framework to identify Mmm antigens involved in interactions with macrophages and targeted by potentially protective antibodies and point towards a pivotal role of complement in the control of inflammatory responses in contagious bovine pleuropneumonia.</p>

Indicate the full web address (DOI or URL) giving public access to these data (if you have any problems with the deposit of your data, please contact contact@microbiol.peercommunityin.org). In case all raw data are included in the preprint, indicate the DOI or URL of the preprint. *

https://doi.org/10.5281/zenodo.7442581You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

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Indicate the full web address (DOI, SWHID or URL) giving public access to these codes (if you have any problems with the deposit of your codes, please contact contact@microbiol.peercommunityin.org). In case all raw codes are included in the preprint, indicate the DOI or URL of the preprint. *

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Keywords (optional)

Contagious bovine pleuropneumonia, Mycoplasma mycoides, macrophages, complement, TNF

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields *

Microbe-microbe and microbe-host interactions

Suggested reviewers - Suggest up to 10 reviewers (provide names and Email addresses). (Optional)

Flavio Sacchini f.sacchini@izs.it, Joachim Frey <joachim.frey@vetsuisse.unibe.ch>, GAURIVAUD Patrice Patrice.GAURIVAUD@anses.fr, Paola Pilo <paola.pilovetsuisse.unibe.ch>, Jose Perez-Casal <jose.perez-casal@usask.ca>, Jochen Meens Jochen.Meens@tiho-hannover.de

e.g. John Doe john@doe.com

No need for them to be recommenders of PCIMicrobiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

Opposed reviewers - Suggest up to 5 people not to invite as reviewers. (Optional)

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Submission date

2022-12-09 15:12:53

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Pablo Zunino

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