PCI Microbiol

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Title *

Bacterial pathogens dynamic during multi-species infectionsuse asterix (*) to get italics

Authors *

Marie-Anne Barny, Sylvia Thieffry, Christelle Gomes de Faria, Elisa Thebault, Jacques PedronPlease use the format "First name initials family name" as in "Marie S. Curie, Niels H. D. Bohr, Albert Einstein, John R. R. Tolkien, Donna T. Strickland"

Year *

2024

Picture *

Abstract *

<p>Soft rot Pectobacteriacea (SRP) gathers more than 30 bacterial species that collectively rot a wide range of plants by producing and secreting a large set of plant cell wall degrading enzymes (PCWDEs). Worldwide potato field surveys identified 15 different SRP species on symptomatic plants and tubers. The abundance of each species observed during outbreaks varies over space and time and the mechanisms driving species shift during outbreak are unknown. Furthermore, multi-species infections are frequently observed and the dynamics of these coinfections are not well understood. To understand the dynamics of coinfections, we set up 16 different synthetic communities of 6 SRP strains to mimic coinfections. The bacteria present in each tested community were representative of 2 different species, with 3 strains per species. These communities were inoculated in potato tubers or on synthetic media and their outcome was followed by amplification and Illumina sequencing of the discriminatory housekeeping gene gapA. We also compared disease incidence and bacterial multiplication in potato tubers during mixed-species infection and single-species infection. A species that was unable to induce disease in potato was efficiently maintained and eventually became dominant in some of the communities tested, indicating that cheating can shape dominant species. Modeling indicates that the cost of PCWDEs production and secretion, the rate of potato degradation and the diffusion rate of degraded substrate could favor the cheater species. Interaction outcomes differed between potato tuber and synthetic medium, highlighting the driving effect of environmental conditions, with higher antagonistic interactions observed in potato tubers. Antagonistic interactions were strain specific and not species specific. Toxicity interference was also observed within some communities, allowing the maintenance of strains otherwise sensitive to toxic compounds. Overall, the results indicate that intraspecific competition, cooperation through trophic interaction and toxicity interference contribute to the maintenance of SRP diversity. The implications of these processes for epidemiological surveillance are discussed.</p>

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https://doi.org/10.5281/zenodo.10212829You should fill this box only if you chose 'All or part of the results presented in this preprint are based on data'. URL must start with http:// or https://

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https://doi.org/10.5281/zenodo.10212829, https://doi.org/10.5281/zenodo.10404740.You should fill this box only if you chose 'Scripts were used to obtain or analyze the results'. URL must start with http:// or https://

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Keywords (optional)

plant, bacteria, evolution, ecology, genomic

Methods that require specific expertise (optional)

NonePlease indicate the methods that may require specialised expertise during the peer review process (use a comma to separate various required expertises).

Thematic fields *

Microbe-microbe and microbe-host interactions, Microbial ecology and environmental microbiology

Suggested reviewers - Suggest up to 10 reviewers (provide names and Email addresses). (Optional)

"Boris Vinatzer" <vinatzer@vt.edu>, "Thomas Pommier" <Thomas.Pommier@inrae.fr>, "Matthieu Barret" <matthieu.barret@inrae.fr>, "Boris Szurek" <boris.szurek@ird.fr>, "Linda Kinkel"<kinkel@umn.edu>, "Jan vanderWolf" <Jan.vanderWolf@wur.nl>, "Amy Charkowski"<amy.charkowski@colostate.edu>, "Robert Czajkowski" <robert.czajkowski@biotech.ug.edu.pl>, Marie Simonin suggested: alain.sarniguet@inrae.fr, Jan vanderWolf suggested: Minna Pirhonen: mpirhone@mappi.helsinki.fi

e.g. John Doe john@doe.com

No need for them to be recommenders of PCIMicrobiol. Please do not suggest reviewers for whom there might be a conflict of interest. Reviewers are not allowed to review preprints written by close colleagues (with whom they have published in the last four years, with whom they have received joint funding in the last four years, or with whom they are currently writing a manuscript, or submitting a grant proposal), or by family members, friends, or anyone for whom bias might affect the nature of the review - see the code of conduct

Opposed reviewers - Suggest up to 5 people not to invite as reviewers. (Optional)

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Submission date

2023-12-12 17:54:07

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Clara Torres-Barceló

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